WSEAS Transactions on Biology and Biomedicine
Print ISSN: 1109-9518, E-ISSN: 2224-2902
Volume 14, 2017
Paper Based Magnetic Nanoparticles Biosensor for Rapid Prostate Specific Antigen Detection
Authors: ,
Abstract: Paper-based magnetic nanoparticle biosensor for the evaluation of active PSA level was designed. This biosensing configuration is based on the detection of PSA amylodic activity using a specific peptide substrate, sandwiched between magnetic nanonanoparticles and gold sensing platform placed on the top of a paper support. Upon PSA addition, an external magnet fixed at the back of the sensor support would accelerate the cleavage of the magnetic nanoparticles-peptide moieties away from the surface. Colorimetric change from black to golden color was visualized by the naked eye. This biosensor was amenable for a qualitative and quantitative PSA detection in buffer and in spiked urine samples with a lower detection limit of 1 ng/mL and 5 ng/mL, respectively. In conclusion, the designed colorimetric biosensor pinpointed superiority in criteria’s including sensitivity; simplicity and fast processing time.
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Keywords: Protease, Prostate Cancer, Prostate Specific Antigen, Colorimetric, Biosenser, Magnetic nano-particles
Pages: 65-68
WSEAS Transactions on Biology and Biomedicine, ISSN / E-ISSN: 1109-9518 / 2224-2902, Volume 14, 2017, Art. #9