WSEAS Transactions on Biology and Biomedicine
Print ISSN: 1109-9518, E-ISSN: 2224-2902
Volume 16, 2019
Effect of Leds on Micropropagated Shoots of Picrorhiza Kurroa Vis-À-vis Biosynthesis of Picroside-i and Key Gene Expression
Authors: , ,
Abstract: Picrorhiza kurroa is a critically endangered medicinal species with various medicinal properties. The present study was conducted in order to see the effect of different lights on the morphological characters of the in vitro grown plants and for the accumulation of Picroside-I compound so that its natural population can be reclaimed by providing these quality rich shoots for industrial demand. The culture conditions for the micro-propagation of P. kurroa were optimised in MS media supplemented with IBA (3 mg/l) and Kinetin (1 mg/l). The novel part of study is use of monochromatic LED’s (Green, Red, Blue and White) in comparison to standardised white fluorescent light for the period of 30 days and in results we observed 4.72 fold increase in number of shoots and shoot area and 100% survival percentage in red and white LED’s as compared to other lights. So this is reported for the first time that LED’s have significant effect on growth and secondary metabolite accumulation. Conditions for the hardening and acclimatisation for in vitro grown plantlets along with LED’s and natural source i.e. sunlight were tested to observe the survival percentage of hardened plants in the greenhouse conditions. HPLC quantification of marker medicinal compound Picroside-I in cultured shoots of P. kurroa have been carried out where 1.458 mg/g and 0.788 mg/g P-I is accumulated in shoots of P. kurroa incubated under white and red LED respectively. G10H gene involved in iridoid pathway of Picroside production showed increase in gene expression by 1.2834 fold in shoots of P. kurroa incubated under white LED as compared to key gene (26S gene) and 1.90 fold expression in shoots of P. kurroa incubated under red LED as compared to shoots incubated under WFL.
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Pages: 90-99
WSEAS Transactions on Biology and Biomedicine, ISSN / E-ISSN: 1109-9518 / 2224-2902, Volume 16, 2019, Art. #11