WSEAS Transactions on Biology and Biomedicine
Print ISSN: 1109-9518, E-ISSN: 2224-2902
Volume 16, 2019
Elicitation of Salidroside Under Tissue Culture Conditions in the Trans-Himalayan Plant Rhodiola Imbricata
Authors: , ,
Abstract: Rhodiola imbricata belonging to Crassulaceae family, is a dioecious perennial plant. It is herb indigenous to the Trans- Himalayan region and exclusively found in Leh-Ladakh valleys of India and border regions of China and Tibet. Various pharmacological activities in R. imbricata viz. hepatoprotective, radioprotective, immunomodulatory etc., are attributed to presence of different phytochemicals such as p- tyrosol, salidroside, rosavin and rosin. But the established plant cell cultures of Rhodiola imbricata usually encounter problems of low product yields and high cost, which discourage its commercialization. So, in order to enhance the secondary metabolite content in R. imbricata, we have studied the effect of different abiotic elicitors on marker compound production. Owing to the previous success of different elicitors on other species of Rhodiola, we have performed chemical and physical elicitations on shoot cultures of Rhodiola imbricata. The best shoot growth in elicited cultures was reported under Photosynthetic light and data were collected for number of shoots 8±0.33 with average shoot size 6±0.63 cm, number of leaves 31±0.16, leaf size 1±0.28 cm and biomass 10±0.96 g. The highest salidroside content 3.117±0.008 mg/g was obtained by using UV light as an elicitor. Along with elicitations, liquid MS media supplemented with different growth hormones have been tried for improving the biomass of R. imbricata under in vitro conditions. The best results were obtained in liquid MS medium supplemented with BAP (1 mg/L) + IBA (2 mg/L) with 87±0.95 g biomass yield in 3 weeks duration of time. The relative enhancement achieved in liquid media in comparison to solid media is 5.35 folds more.
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Pages: 75-89
WSEAS Transactions on Biology and Biomedicine, ISSN / E-ISSN: 1109-9518 / 2224-2902, Volume 16, 2019, Art. #10