wseas-12211-20261015053117-881570 20261015053117953 wseas/wseas wseas.group@gmail.com WSEAS Molecular Sciences and Applications 2944-9138 2732-9992 05 14 2026 05 14 2026 6 Paata J. Kervalishvili Department of Physical Sciences, Tbilisi, GEORGIA This study develops a multiphysics framework for evaluating whether alternating magnetic fields (AMF) applied to Fe₃O₄ and MnFe₂O₄ nanoparticles can modify ATP hydrolysis through thermal, mechanical, and ion-mediated pathways. Rather than assigning a single phenomenological magnetic activation-energy reduction to all effects, the model starts from Arrhenius/Eyring kinetics and derives the observed rate change as the sum of a temperature-dependent contribution and explicitly defined non-thermal perturbations. The thermal term is constrained by the measured temperature history and nanoparticle specific absorption rate (SAR); the mechanical term is related to magnetic torque, hydrodynamic damping, and experimentally measurable particle motion; and the catalytic term is expressed through independently measurable Fe²⁺/Mn²⁺ concentrations and dose-response parameters. The available experimental observations reported in the original manuscript—2.5–3-fold enhancement of non-enzymatic hydrolysis under nanoparticle/AMF conditions, approximately 40% enhancement of Na⁺/K⁺-ATPase activity under stated isothermal conditions, and Raman/FTIR changes in phosphate-associated bands—are retained, but their interpretation is deliberately narrowed. In particular, the present data support rate enhancement under specified conditions and are consistent with multiple mechanisms; they do not, by themselves, establish a unique mechanical mechanism, nanoscale temperature gradients, or synergistic coupling. A mechanism-separation experimental matrix is therefore defined, together with quantitative spectroscopic observables and statistical requirements. 10 15 2026 10 15 2026 114 10 https://creativecommons.org/licenses/by/4.0/ 10.37394/232023.2026.6.10 https://wseas.com/journals/articles.php?id=12211 Alberts, B., Johnson, A., Lewis, J., Raff, M., Roberts, K., Walter, P. Molecular Biology of the Cell. 4th edn. Garland Science, New York (2002). Kervalishvili, P.J. Vibration and resonance spectroscopy—a tool for investigation and activation of adenosine triphosphate molecule. In: Annual Medical Conference Proceedings, Euroscience-Georgia, pp. 15–22. Tbilisi, Georgia (2023). Dunn, J., Grider, M.H. Physiology, adenosine triphosphate. In: StatPearls. StatPearls Publishing, Treasure Island, FL (2023). Vander Heiden, M.G., Cantley, L.C., Thompson, C.B. Understanding the Warburg effect: the metabolic requirements of cell proliferation. Science 324, 1029–1033 (2009). Pankhurst, Q.A., Connolly, J., Jones, S.K., Dobson, J. Applications of magnetic nanoparticles in biomedicine. J. Phys. D: Appl. Phys. 36, R167–R181 (2003). Gupta, A.K., Gupta, M. Synthesis and surface engineering of iron oxide nanoparticles for biomedical applications. Biomaterials 26, 3995–4021 (2005). Rosensweig, R.E. Heating magnetic fluid with alternating magnetic field. J. Magn. Magn. Mater. 252, 370–376 (2002). Laurent, S., Forge, D., Port, M., Roch, A., Robic, C., Vander Elst, L., Muller, R.N. Magnetic iron oxide nanoparticles: synthesis, stabilization, vectorization, physicochemical characterizations, and biological applications. Chem. Rev. 108, 2064–2110 (2008). Kervalishvili, P.J. Ferromagnetic nanosystems in biomedicine—some examples. Journal of Social, Ecological & Clinical Pediatrics 27, 25–32 (2025). Bonora, M., et al. ATP synthesis and storage. Purinergic Signalling 8, 343–357 (2012). Johnson, L.N. The effects of phosphorylation on the structure and function of proteins. Annual Review of Biophysics and Biomolecular Structure 22, 199–232 (1993). Berberashvili, T., Gotsiridze, I., Gurtskaia, Z., Kervalishvili, P. Laser Based Optical Spectrometry Studies of Molecular Mechanisms in ATP Reactions. Book of Proceedings of the International Workshop on the Physics and Technology of Laser-Induced Plasma for Green Energy and Biomedical Applications, Japan–Georgia–Europe, 2–4 October 2025, pp. 63–70. Technical University, 2026. Berberashvili, T., Kervalishvili, P. Ferromagnetic Nanosystems and Magnetically Activated ATP Reactions: A Pathway to Cellular Energy Manipulation. International Conference “Advanced Materials and Technologies” (ICAMT 2025), Tbilisi, Georgia, 15–17 October 2025.