Abstract: - The aim of this study was to evaluate the effect of ROS on the intensity of oxidative and energy

metabolism in the blood of rats with an operational model of ischemia of the dorsal skin flap in vivo. Our

studies have allowed us to establish that the modeling of an extensive skin defect is accompanied by

pronounced shifts in oxidative metabolism in the blood plasma of animals. At the same time, in the absence of

pathogenetic treatment, signs of oxidative stress are formed, including the intensification of free radical

processes and the inhibition of general antioxidant activity in combination with the accumulation of an

increased amount of lipoperoxidation products (in particular, malondialdehyde). Conducting experimental

therapy with the introduction of ozone or treatment with electromagnetic radiation of the EHF band allows

partially compensating for these metabolic disorders, however, the most optimal option is a combination of

these factors within a single scheme. It should be noted that the results obtained are of great applied importance

Despite significant achievements in the field of

tissue engineering, cellular technologies, and

regenerative medicine, the problem of adequate

restoration of full-fledged skin in case of injury

remains relevant. Therefore, it is expedient to search

for and evaluate the effectiveness of new methods

for stimulating reparative skin regeneration, [1], [2].

Taking into account the fact that one of the most

dorsal skin flap, it is advisable to use a regulator of

the state of pro- and antioxidant systems, which can

be reactive oxygen species (ROS), including ozone,

[7], [8], [9].

medicine is associated with its high redox potential,

in living biological systems, including the influence

of these factors on the body at the molecular,

cellular, and systemic levels, [13], [14]. Therefore, it

is important to study the mechanisms of action of

ROS on the functional state of the pro- and

antioxidant systems of the body, and the activity of

oxidoreductases under conditions of experimental

oxidative stress in vivo.

In addition, the effects of a sanogenic combined

source of ROS (including medical ozone) and the

reflection of the consequences are not fully applied.

This study aimed to evaluate the effect of ROS on

the intensity of oxidative and energy metabolism in

the blood of rats with an operational model of

ischemia of the dorsal skin flap in vivo.

In the experiment, 25 male rats of the Wistar line

European Convention ET/S 129, 1986, and

Directives 86/609 ESC, [15]. The animals were

divided into 5 groups: group 1 – intact (healthy rats,

n=5), group 2 – control – operated animals without

any effects (n=5), and Group 3 - experimental–

operated animals with ROS treatment in the

postoperative period (n=5). Animals of the fourth

(experimental) group (n=5) were irradiated daily

(once a day for seven days) with a modified device

"Amphit-0,2/10-01", [16], [17], with a noise

radiation and ozone therapy procedures.

performed during the study. In rats of the

experimental and control groups on the depilated

back under

flap on a feeding leg with an axial type of blood

severity of LPO in erythrocytes. To assess the

intensity of LPO, the level of the secondary product

of free radical oxidation – malonic dialdehyde

(MDA) in plasma and erythrocyte hemolysate was

determined (1:10), [19].

of the antioxidant defense system, the activity of

superoxide dismutase (SOD), which converts the

superoxide radical into the electroneutral form of

spectrophotometric method was used based on

determining the rate of decomposition of H2O2 by

catalase of the test sample with the formation of

water and oxygen, [21].

to the Statistica 6.0 program, with the help of which

the arithmetic mean of the indicators and the error

of the average were calculated. The significance of

the differences between the indicators was

determined using the student’s t-test. Differences at

free radical processes (p<0.05; Fig. 1). This creates

prerequisites for the development of secondary

oxidative damage to cells and tissues that occurs in

response to the presence of an extensive defect of

the skin.

was recorded in this mode, it can be argued that it

makes it possible to realize the most complete

antioxidant effect.

the secondary product of lipoperoxidation –

malondialdehyde – in the blood plasma of rats of the

formed groups (Fig. 3). It was found that only in

operated animals receiving exclusively basic

treatment, there was an increase in this indicator

the intensity of free radical processes and the

antioxidant potential of the biological fluid.

Therefore, such an option, including simultaneous

courses of EHF therapy and ozone therapy, provides

the maximum antioxidant systemic effect.

[3] A. V. Vorobyov, A.K. Martusevich, A.G.

[4] E.B. Menshchikova, V.Z. Lankin, N.K.

[6] N.P. Chesnokova, E.V. Ponukalina, M.N.

[7] A.A. Martusevich, A.G. Solovieva, A.K.

[9] J. Parva, P. Gunjan, Y. Priti, Ozone therapy:

[10] J. Zeng, J. Lu, Mechanisms of action involved

[11] M.N. Mandhare, D.M. Jagdale, P.L. Gaikwad,

-Andrew K. Martusevich: Conceptualization,

Formal analysis and Writing – original draft.

-Irina E. Sazonova: Investigation, Formal analysis

and Writing – original draft and Writing – review &

editing.

-Anna G. Soloveva: Investigation, Formal analysis

This research work was supported by the Ministry

of Health of the Russian Federation.

The authors have no conflicts of interest to declare.

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