sublingual vein was performed. The animals of the second (control) and third (main) groups under combined

anesthesia were subjected to thermal trauma according to our methodology, standard local treatment was

carried out. Starting from the day following the injury, the rats of the main group were inhaled daily for 10 days

of the airflow coming from the singlet oxygen generator. Blood samples from rats of all groups were collected

and tested for crystallogenic properties and crystallogenesis-inducing activity. It was found that the inhalation

of singlet oxygen in animals with severe thermal trauma contributes to the partial normalization of the

crystallogenic activity of blood serum, which positively characterizes its rehabilitation potential. This trend

must take place when assessing both the intrinsic crystallogenic and initiating properties of biological fluid,

which is manifested both in the dynamics of morphological and morphometric indicators and the optical

characteristics of serum facies.

containing singlet oxygen are perceived by several

domestic and foreign researchers as a means of

rehabilitation, contributing to an increase in the

and deciphering the effects and mechanisms of the

influence of singlet oxygen on biological systems in

physiological and pathological conditions. Thus,

human blood samples have shown that its treatment

with singlet acid promotes the stimulation of

antioxidant systems, energy metabolism of

erythrocytes, and optimization of acid-base

equilibrium parameters, [4]. In healthy animals, a

ten-day course of singlet oxygen inhalation also

caused an antioxidant effect and activation of

the example of thermal trauma) positive metabolic

effects of singlet oxygen have also been established,

while the effect on the dehydration structuring of

blood serum has not been studied.

dynamics of the crystallogenic properties of rat

blood serum during a course of singlet oxygen

inhalation in the post-burn period.

ESC. The first group (n=10) was intact, no

manipulations were performed on the animals

included in it, but only a single blood irradiation

from the sublingual vein was performed.

(main) groups under combined anesthesia ("Zoletil"

+ "Xyla vet") were subjected to thermal trauma

according to our methodology, standard local

treatment was carried out, [7]. Starting from the day

following the injury, the rats of the main group were

the completion of the full course of inhalations in

rats of this group, blood samples were obtained

from the sublingual vein for examination. At the

same time, blood was taken from animals of the

second group.

suppression of the crystallogenic activity of the

biological environment, simplification of the

composition of elements, and a sharp increase in the

number and degree of destruction of the destroyed

elements, as evidenced by shifts in the levels of

crystallizability, the index of structure and the

degree of destruction of facies, respectively. At the

same time, the size of the marginal zone of the

micropreparation in burned animals was

significantly reduced, which is realized in a change

biological fluid is evidenced by a sharp drop in the

level of crystallizability and structural index in rats

with thermal trauma compared with intact animals

(p<0.05 for both indicators). Against this

background, the processes of structure-building in

drying samples of the biological environment are

disrupted, as evidenced by a significant increase in

the degree of destruction of the crystallogenesis

facies (p<0.05 in relation to healthy rats) – the main

criterion of "correctness", [6]. Hypoproteinemia and

dysproteinemia formed in burnt animals are

reflected in a decrease in the relative width of the

marginal zone (p<0.05) containing proteins of the

native structure and estimated by the clarity of

marginal zone, [9], [10], [13], [14], [15].

facia destruction degree, Mz – clarity of the

marginal zone)

significantly reduces the severity of pathological

shifts in the crystallogenic properties of rat blood

serum (Figure 1). It should be emphasized that the

second of the above parameters after a course of

singlet oxygen injections was found to be only

slightly reduced in comparison with healthy animals

(p<0.1). At the same time, the high severity of

dysmetabolic shifts induced by combined thermal

trauma inflicted on rats contributed to the

preservation of a significantly reduced level of the

tezigraphic index, as well as a moderate tendency to

optimize the degree of destruction of facies after a

course of treatment supplemented by inhalation use