A differential mathematical model for experiments to determine the
efficacy of treatments against the bean weevil
PETRU CARDEI
Computer Engineering
INMA
6 Ion Ionescu de la Brad Blvd., Sector 1, Bucharest, 013813
ROMANIA
CONSTANTINA CHIRECEANU
Research and Development Institute for Plant Protection Bucharest, 8 Ion Ionescu de la Brad Blvd.
ROMANIA
Abstract: - The article presents a mathematical model for experiments evaluating the effectiveness of
diatomaceous earth treatments against the bean weevil, Acanthoscelides obtectus. The proposed mathematical
model is of the differential type, inspired by the category of prey-predator models. The system of equations is
nonlinear and is solved numerically. A systemic characterization of the bean weevil treatment process is used to
describe the model, which uses three functions of time: the number of beans, the pest population, and the
amount of diatomaceous earth. The three functions offer users four applications: forecasting, control,
formulation of treatment efficacy estimators, and simulation of different types of pest control. The model is
built for closed (isolated) experiments typical of laboratories, but this feature makes it extensible to other
treatments to combat bean weevils in closed spaces characteristic of the storage of beans in silos.
Key-Words: -mathematical model, bean weevil, treatments, experiences, efficacy, control
Received: April 13, 2022. Revised: December 12, 2022. Accepted: January 7, 2023. Published: February 14, 2023.
1 Introduction
The mathematical modelling of experiences in
biological processes is a broad field that has
received attention in the literature.Numerous articles
deal with the general theory of models and
(mathematical) modelling of biological systems [8],
[9], [10], [11], [12], and [38]. These are just a few
titles from a large number of papers on the subject.
The computer simulation of the integrated
management of spruce worms was already used in
1982 [26].
Many concerns similar to the ones described in this
paper are also found in the literature. In [13], the
authors mathematically model the control of
harmful insects by interrupting mating and capturing
them, also using a system of differential equations.
Concerns in the field of strategy for the
development of mathematical modelling and
experimentation in the field of biology are found in
[14], some of them resulting from our work.
A broad differential model (with fifteen differential
equations), which, as in our case, uses the
calibration operation, is dedicated to a deadly
tropical disease for humans[17]. Differential models
for mosquitoes carrying Wolbachia bacteria and
dengue disease are reviewed in [18]. A new
differential model is presented in [19] for the study
of the dynamics of vector-borne disease
transmission (a new mathematical model for the
transmission dynamics of vector-borne diseases
with vertical transmission and cure is developed).
Differential models of prey-predator types with
applications in agricultural crop protection are
exposed in [21]. The authors [22], [39] used simple
mathematical models to describe the dynamics of
interaction between sterile and wild insect
populations in the insect control technique by
launching the sterile male Sterile Insect Technique
(SIT). Similar problems are studied by the same
authors in [23], [25].
Differential models and mixed differential and
partial derivative models for the description and
understanding of malaria spread are presented in
[20]. Another category of mathematical models is
those that combine statistical models with
differential ones, possibly using statistically
modelled functions in the dynamic, differential
model [15]. Statistical models have long been used
in the study of insect populations [24]. The use of
even more complex, semi-discrete mathematical
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models is part of a class of hybrid models, which are
often described by continuous dynamics but
repeatedly experience discrete changes at certain
times [16].
Concerns at a higher-level aim at an optimal design
of the dynamic models of the pest populations [27].
Works dedicated to determining the efficacy of
alternative treatments, even for stored beans and,
among others, diatomaceous earth, using statistical
processing of experimental data can be found in
[28], [30], and [32]. Statistical mathematical
modelling of experimental data on the efficacy of
diatomic soils against insects can be found in [32],
[33], [34], [35], and [36]. A mathematical model for
describing the kinetics of diatomic soil adhesion on
the body of the bruchids Callosobruchus maculatus
(F.) or Acanthoscelidesobtectus (Coleoptera:
Bruchidae) is proposed by the authors [29], [31]. In
[37], the authors also consider the influence of
ambient temperature and humidity on the bean
weevil population.At the level of descriptive
statistics, the results can be used to extend
mathematical models to the two variables, which in
the present research are considered constants.
Mathematical models are constructed to explain the
phenomena of the physical world better than purely
descriptive models. A more important role of
mathematical models is to make predictions about
the values of the parameters of the modelled
phenomenon. A higher level for a mathematical
model is reached when it can demonstrate the
existence of optimal values for the parameters of the
phenomenon and, in addition, calculate these values.
Based on mathematical models, the authors [40]
optimize the control of mosquito populations.
Several mathematical models with which heuristic
optimizations are performed are reviewed by the
authors [41]. Mathematical modelling with control
and optimization also exposes the authors [42] to
sugar beet pests. The articles of the authors [43],
[44], [45], [46], [47], and many others are inscribed
on the same coordinates.
2 Material and method
The subject of the research described in this article
is the mathematical modelling of the behaviour of a
population of bean weevils in a closed or isolated
system. The purpose of the model is to estimate the
effectiveness of the treatment.
2.1 Material of the study
The physical support of the study consists of the
closed system made in a cylindrical container with
transparent walls, in which there are three main
components: beans, adult bean weevils, and
diatomaceous earth as a treatment against them.
2.2 Material of the study
The method of mathematical modelling of the
biological process that constitutes the study material
is the description of the system as a dynamic system
of the prey-predator type with three components.
The differential mathematical model that is
presented in this paper, tries to describe the
behaviour of a closed system, consisting of three
components: a prey and two predators. Prey (stored
beans) is the food for the first predator (the bean
weevil), and the substance with which the treatment
is performed against the bean weevil is considered
to be the second predator. The closed system
consisting of bean grains, bean weevils, and bean
weevil control substance (in this case, diatomaceous
earth) is enclosed in glass jars with transparent walls
and a perforated lid to allow ventilation (jars with a
special locking system; see Figs. 1 and 2) [1] and
[2].
Fig. 1 Isolated systems: the beans, the beans weevil,
and the fighting substance.
Fig. 2 Isolated systems: prey, primary predators, and
secondary predators.
The exchange of air and humidity between the
insulated system and the room in which the system
is stored will be neglected.
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The behaviour of the components of the closed or
isolated system described above allowed us to
formulate the following hypotheses, which were
used in the mathematical modelling of the system:
i1) The prey (beans) interacts only with the primary
predator (bean weevil);
i2) The prey does not record its growth or
reproduction;
i3) The primary predator (bean gargoyles)
interacts for its own feeding with prey and
negatively, for combat, with the secondary
predator (combating substance);
i4) The study period is limited to exactly one
reproduction cycle, so natural growth and mortality
exist but are hidden by the action of the control
substance (a secondary predator);
i5) The growth rate of the secondary predator
(control substance) is zero (it does not reproduce),
and its "mortality" is its consumption rate in the
control action.
2.3 The system of differential equations
The system of differential equations that models the
isolated process of fighting the bean weevil, in
hypotheses i1)-i5), is given in (1).
󰇛󰇜
 󰇛󰇜
󰇛󰇜
 󰇛󰇜󰇛󰇜󰇛󰇜󰇛󰇜
󰇛󰇜󰇛󰇜󰇛󰇜,

 󰇛󰇜󰇛󰇜󰇛󰇜
(1)
with the initial conditions:
(2)
The definition, significance, and units of
measurement of the variables and parameters of the
model (1)-(2) are given in Table 1.
The natural mortality function of bean weevils is
modelled after [3], with the expression (3).
󰇛󰇜
󰇡
󰇢.
(3)
For the consumption function of the diatomaceous
earth in the control process, the hypothesis is (4).
󰇛󰇜

(4)
and for the function of the interaction between the
bean weevil population and the diatomaceous earth,
hypothesis (5) is used.
󰇛󰇜

(5)
Table 1 Mathematical model parameters (1)-(2),
meaning, notation, and units.
Definition
Notation
Units
The mass of uninfected beans at the
current time
kg
The mass of the bean weevils at the
current time
kg
The mass of diatomaceous earth
available at present
kg
Bean mass at the initial time
kg
The mass of bean weevils at the initial
time
kg
Diatomaceous earth mass at the initial
time
kg
The initial time
days
Parameter of the bean damage
consumed by the bean weevil
population
days-1
Parameter of interaction between the
bean, and the bean weevil population
days-
1kg-1
The natural mortality function of the
bean weevil
󰇛󰇜
days-1
The interaction function between the
bean weevil population and the
diatomaceous earth
󰇛󰇜
days-1
kg-1
Diatomaceous earth consumption rate
in the control process
󰇛󰇜
days-1
Diatomaceous earth supply rate during
the process
󰇛󰇜
kg
days-1
The period of the bean weevil
reproduction cycle
days
The consumption ratio of
diatomaceous earth during the process
days-1
The constant interaction between the
bean weevil population and the
diatomaceous earth
days-1
kg-1
The average mass of the beans
kg
The number of beans placed in each
glass jar
nb
-
The average mass of a bean weevil
mg
kg
The number of bean weevils placed in
each glass jar
-
Dose of diatomaceous earth
administered

-
Mortality function of the pest
population
󰇛󰇜
%
The function of the percentage of
infested grains
󰇛󰇜
%
Percentage function of diatomite
available
󰇛󰇜
%
Percentage mortality function of the
pest population at the c dose of
diatomite
󰇛󰇜
%
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2.4 Estimation of the constant parameters
involved in the model
The mass of the beans with which the experiments
described in [1] were carried out was calculated by
averaging 1000 grains, which together weighed 145-
450 g. Therefore, we work with the average mass of
a bean having the value = 0.0002975 kg. For
each cylindrical container (glass jar) that isolates the
experiment (control process), a number (200 -
700 beans/ glass jar). As a result, the average initial
mass of beans in each experiment is approximately
= 0.02975 kg. The bean weevils were weighed,
and it was found that 50 adult weevils weigh an
average of 0.1176 g, therefore, the mass of a bean
weevil is = 0.000002352 kg. As for each
experiment, 50 weevils were introduced into
containers; the result is the initial mass of bean
weevils, which is = 0.0001176 kg. The initial
dose of diatomaceous earth was limited between
100 and 900 ppm (by mass ratio to the initial mass
of beans). As a result, the initial mass of diatomite,
, is between 0.0000001 and 0.0000009 kg,
calculated according to the formula:.
The value of the parameter is inspired by [4],
assuming that during the experiment, about 23% of
the bean mass is infested. It took = 17 days-1 to
achieve a percentage of approximately 77% beans
remaining uninfected. For the period of the bean
weevil reproduction cycle, the value = 14 days
was taken, in the conditions in which the
temperature in the experiment container is kept
constant. For the first tests, it will be assumed that
the pest control operation is done only by the initial
dose of diatomite and that no additional amounts are
added during the 14 days of the experiment.
Therefore, the function 󰇛󰇜 is identical to zero. The
parameter , which describes the consumption of
diatomaceous earth, is used only to control the
amount of diatomite consumed in the control
operation.
Only three model constants remain to be identified:
the constants that define the functions , , and .
We currently lack special experiences of direct or
indirect determination to set the values of these
parameters. For this reason, a calibration process is
used using the data from the experiments described
in [1]. The experimental data we rely on are the
mortality rates achieved by the treatments with
doses of 100, 300, 500, and 900 ppm at observation
times of 3 and 7 days. Let the experimental
mortality  and , = 1, ..., 4, correspond to
the four doses of diatomite applied, at times of 3 and
7 days, respectively. The system of differential
equations (1), with the initial conditions (2), is
solved numerically starting from randomly
established starting values (by tests that tend to
approach the experimental values at times of 3 and 7
days). For each pair of values 󰇛󰇜,
corresponding to the coordinate network taken into
account in the calibration, the sum is calculated
using the equations (6) and (7),
󰇛 󰇜󰇛 󰇜

(6)
where:
 󰇛󰇜


(7)
data obtained for each run = 1,2,..., m (number of
calibrations runs required; we use 20). The
optimal torque will be that for which the sum is
minimal, after = 1, 2,..., m. Using this model
calibration procedure, based on a heuristic
optimization procedure [5], the following values are
obtained: = 0.045 days-1kg-1 and = 4925 days-
1kg-1.
In the same calibration process, the constant was
calibrated by heuristic optimization so that at the
diatomite concentration of 900 ppm, the bean weevil
population disappeared. Initially, each jar contained
50 bean weevils; the value 0.00005 was chosen for
because the percentage mortality is greater than
98%.
3 Results
Using the values of the parameters of the model (1)-
(2), chosen in 2.4, one can find the behaviour of the
system: beans-weevils-diatomaceous earth. The
system is isolated in containers such as those in
Figs. 1 and 2.
3.1 Validation of the model in the
experimental case
The time dependence of the percentage
mortality of the bean population is shown in
Fig. 3 for the values of system parameters
experimentally determined and specified in 2.4.
The percentage mortality function, represented
graphically in Fig. 3, is calculated starting from the
population 󰇛󰇜, resulting from solving the
differential problem (1)-(2) and is given in (8).
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󰇛󰇜󰇛󰇜
(8)
The percentage of infested grain is calculated from
the solution of problem (1)-(2), according to the
formula (9).
󰇛󰇜󰇛󰇜
(9)
Fig. 3 Dependence of mortality percentage on
time for four doses used experimentally.
Fig. 4 The percentage of infested beans varies
with time for four experimental doses.
The available diatomite function is calculated using
the solution to the problem (1)-(2), according to the
formula (10).
󰇛󰇜󰇛󰇜
(10)
Fig. 5 The time dependence of the percentage of
available diatomaceous earth for four
experimental doses.
3.2 Applications
3.2.1 Process prediction and control
The simplest application of model (1)-(2) is the
prediction of the results of the experiment for
treatment doses different from those used in the
physical experiments and in the calibration of the
model. The principles of interpolation recommend
that we do not use doses outside the experimental
range. If, however, we use a dose outside the
experimental working range ([100,900] ppm), quite
far from the maximum limit (for example, 2000
ppm), the response of the model is that of Fig. 6.
Fig. 6 The time dependence of the three
characteristic functions of the control process
carried out in an isolated environment at a dose
of diatomite with a value of 2000 ppm.
This result is subject to a possible validation of the
model. The forecast shows how many days are
needed for the pest population mortality rate to
reach the conventional critical value (for example,
99%), depending on the treatment dose chosen. In
other words, we can select a dose that shortens the
time required to achieve the desired mortality or,
implicitly, a dose that reduces the damage to stored
grains (curve of the quantity of infested grains).
The model also allows control of the amount of
diatomaceous earth still available for action against
pests.
3.2.2 Efficacy of treatment
Using the results of the mathematical model (1)-(2),
several functions can be defined that measure the
efficacy of the treatment. These are defined using
the functions provided by the solution to problem
(1)-(2).The two efficiency indices that will be
defined in this chapter are based on the percentage
mortality index (Fig. 7).
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Repellence index
The repellence index function is defined by [6]
according to the formula (11).
Fig. 7 The dependence of the mortality rate on time,
for five doses of treatment, and the zero-dose
corresponding to the control experiment.
󰇛󰇜󰇛󰇜
󰇛󰇜󰇛󰇜󰇛󰇠
(11)
An average repellence index can be defined
according to the formula (12).
󰇛󰇜
󰇛󰇜

(12)
where is a very small number, for example, 1% of
added to the initial moment in formula (12), so
that the repellence index function makes sense on
the whole mediation range. The repellence index
(12) is a dose-dependent number , see Fig. 8. The
mortality function is a function of two variables,
and , but its consideration in the model will
change the model in the future into one with a
partial differential equation.
Fig. 8 Time dependence of repellence indices (11)
for the four doses of diatomaceous earth used in the
experiments.
The Abbott indexes
A measure of efficacy can be defined starting with
Abbott's formula [7]. The efficacy function inspired
by the source [7] is defined by the formula (13).
󰇛󰇜󰇛󰇜󰇛󰇜
󰇛󰇜󰇛󰇠.
(13)
As with the repellence index, an average Abbott-
type efficacy can be defined, which is a number that
depends on the dose of the treatment substance (14).
The dependence of the Abbott index on time can be
seen in Fig. 9.
󰇛󰇜
󰇛󰇜
.
(14)
Similarly, other measures of the efficacy of pest
treatment can be introduced.
The average values of the repellence indices,
according to the formula (12), are: 󰇛󰇜
󰇛󰇜󰇛󰇜 and
󰇛󰇜, taking  days.
Fig. 9 Time dependence of Abbott-type efficacy for
the four experimental treatment doses.
The average values of the corresponding Abbott
efficacies, according to formula (14), are: 󰇛󰇜
󰇛󰇜󰇛󰇜󰇛󰇜
, taking  day.
3.2.3 Additional loads with treatment substance
Sometimes, the initial amount of substance with
which the treatment is done may not be enough to
achieve the desired efficacy within the time limit.
To increase the efficacy of the treatment, an
additional loading of the pest control substance
(diatomite, in this case) can be performed, an
operation that model (1)-(2) can simulate through a
󰇛󰇜 function that has positive values. The
simulated load in this example has the expression
from the equation (15).
󰇛󰇜󰇫󰇟󰇜󰇛󰇠

󰇟󰇠.
(15)
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The numerical analysis used the following data:
 ppm,  kg,
which corresponds to a uniform load on the third
day of the experiment with a loading speed of 70
ppm/day.
The function of loading the pest with the attack
substance is shown in Fig. 9. For this method of
combat, the response of the mathematical model
of the system described above is graphically
presented in Fig. 10.
Fig. 10 Time-dependence of the amount of
diatomaceous earth and the loading speed during the
process.
Fig. 11Time-dependence of percentage mortality for
initial loading experiments with doses of 100, 300,
500, and 900 ppm, compared to the percentage
mortality in the control sample with zero initial
loadings and loaded on the second and third days, as
shown by the curve 󰇛󰇜 of Fig. 10.
4 Comments
The mathematical model proposed in this article
manages to simulate the main aspects of the process
of experimental estimation of the efficacy of
treatments against pests.
The shape of the mortality curves is in agreement
with the experimental data, at least in the area of
intermediate control times. Differences occur at low
doses of pest control substances in the final part of
the time interval, where mortality does not reach
100% (see Fig. 3). This behaviour can be further
studied, possibly altering the natural mortality of the
bean weevil population, possibly aided by a greater
number of experiments, with a much better
resolution over time, in particular.
The percentage mortality curves are ordered by the
doses of substance used and are directly correlated
(see Figs. 3, and 7). The curves of the infested bean
percent are also ordered by the doses of pest control
substance used, but the correlation is negative, i.e.,
the percent of infested beans increases as the dose of
diatomite decreases (see Fig. 4). The curves of the
percentage amount of diatomaceous earth available
in the process appear in Fig. 5. They are ordered in
reverse order of the dose of substance applied. The
graphs in Fig. 5 must be read carefully because the
curves included are of a percentage nature, but the
quantities of diatomaceous earth from which they
start (the initial load) are different for each
concentration. These statements are validating
elements of the model (i.e., (1)-(2)) at the current
level of the mathematical model.
Several applications present the capabilities of the
mathematical model presented in Chapter 3.2. The
prognosis and control of the process are presented in
Chapter 3.2.1. This application shows the predictive
role of function (mass of the pest population) and
the control role of functions (mass of uninfected
beans) and (mass of diatomaceous earth
available). On a single graph, the variation in time
of the three functions in a pest control process with
the initial loading of diatomaceous earth can be seen
in Fig. 6. The applications in chapter 3.2.2 provide
examples of measures of the efficacy of some
treatments. There are current efficiencies (time-
dependent functions), but there are also global
efficiencies, which are numbers. Both efficacy types
depend on the dose of substance used for the
treatment of bean weevils. To understand the
behaviour of efficacy functions, the time
dependence of mortality functions for four doses of
diatomite is represented in Fig. 7, together with the
natural mortality curve (control or zero treatment
doses). Starting from these results and applying
formulas (11)-(14), the efficacy of the treatments is
obtained: the repellent efficacy function and the
Abbott type efficacy function, respectively, and the
average repellence and Abbott type indices. All
indications show the same ranking of treatments
with the same control substance, with the place
occupied by each treatment being proportional to
the dose administered.
A third application, presented in Chapter 3.2.3,
demonstrates the ability of the mathematical model
to describe treatments with additional loading of the
treatment substance. The system response in this
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case of loading in terms of pest mortality is given in
Fig. 11, and the evolution in time of the additional
load and the loading speed is given in Fig. 10. It is
observed that starting treatment at a lower dose and
supplementing with the active substance along the
way may increase the efficacy of treatment at a
higher initial dose. In [29], interpolation curves
similar to those of Figs. 7, 9, and 11 appear in
connection with the accumulation of diatomic soils
per individual bean weevil, separately for males and
females. These similarities show that there are
strong links between the individual accumulation of
diatomaceous earth and the percentage mortality,
which a possible development of the model could
highlight. Substance use for treatment would thus be
more deeply embedded in the mathematical model,
describing the phenomenon and interpreting the
results.
The mathematical model of experiments to estimate
the efficacy of substances for controlling bean
weevils can be extended to common storage spaces,
such as silos. In this case, it must be taken into
account that during the storage time there may be
fluctuations in the loading with stored material, and
the loading with substances to control pests varies
over time. Application 3.2.3 demonstrates that the
proposed mathematical model can simulate such
phenomena. To obtain a minimal model of the silo
treatment processes, a loading (or unloading) term
with stored material must be added to the right
member of the first equation in (1), similar to the
term 󰇛󰇜 in the third equation of the same system.
Also, both due to the real climatic conditions and
potential treatments with thermal or humidity
shocks, we will look in the future for an extension
of the mathematical model proposed in this paper,
which will contain the two variables, as some
authors have conceived already [37] and [55].
The mathematical model proposed in this paper
goes through the three stages indicated in a
reference paper [8]: "conceptualization of the
biological system into a model mathematical
formalization of the previous conceptual model and
optimization and system management derived from
the analysis of the mathematical model." Because
the answer of the model proposed in this paper has
main elements of similarity with the real isolated
system of experiments to estimate the efficacy of
treatments against pests (the shape and values of
mortality curves), it is considered that the model is
at least partially correct and relevant. These
conclusions are important in connection with
Manfred Eigen's statement in The Origins of
Biological Information: "A theory has only the
alternative of being right or wrong. A model has a
third possibility: it may be right, but irrelevant."
Percentage mortality curves, similar in shape to
those provided by the model proposed in this paper,
are frequently present in the results in the literature
[48] and [49]. The experimental curves exposed in
[1] and [2] are often found as forms in the literature
[50], [51], and [52]. Especially regarding the bean
weevil, such mortality curves are found in [53],
[54], and [55].
5 Conclusion
The mathematical model presented in the article
satisfactorily simulates the treatment of beans
attacked by bean weevils using diatomaceous earth.
The effects highlighted are generally consistent with
the experimental results in the literature. A system
of three differential equations inspired by
mathematical models of predator-prey types
describes the process of treating beans against
bean weevil.
The main applications of the model consist of the
prediction and control of the treatment, the
formulation of some measures of the treatment’s
efficacy, and the simulation of some processes with
intermediate loads in time. The last application
opens a wide field of applications related to
treatments against pests that act on the storage
spaces of agricultural materials (e.g., silos), spaces
considered as isolated systems, but with inputs and
outputs in time of the stored material and substances
for pest treatment.
As an important direction of model development, it
is proposed to introduce the influence of
temperature (if it proves effective, including
humidity), because there are treatments against bean
weevils that involve lowering the ambient
temperature to a certain value for some time.
All directions of model development can be done
only to the extent that we have sufficient
experimental data (as many direct ones as possible),
at a temporal resolution of at least one day, and at a
longer range of values for concentrations, which is
also valid for temperature (a continuous or very
high-resolution monitoring action would be
desirable).
The utility of the model results mainly from the
possibility of predicting the achievement of the
desired mortality at a certain time and the dose of
substance to control pests so that the desired
mortality occurs at a certain time. Another
application of the proposed mathematical model
refers to the optimal character of the desired
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DOI: 10.37394/23208.2023.20.2
Petru Cardei, Constantina Chireceanu
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Volume 20, 2023
mortality at the prescribed time. This is to be
obtained through the precise selection of the
combating substance dose so that there is no
waste or risk to not achieving the prescribed
time or pest mortality. The measures of the
efficacy of the elaborated treatments are also useful
and start from the answer of the model, which
allows reaching the main purpose of the modelling:
the simulation of the experiences in an isolated
system for estimating the efficacy of the treatments.
The indications and clarifications resulting from
solving the model regarding the intensive and
extensive development of experiences required to
deepen the problem of simulation and sorting
treatments against pests are also useful for future
work.
A specification of principle for the possible
continuation of the study refers to the natural
approach to the pest control phenomenon described
in this article. We believe that a new approach to the
problem of the efficacy of treatments against pests
must begin with modelling the normal life of the
pest and increasing and decreasing the insect
population in the absence of treatments. After this
model is close enough to the experimental reality,
functions can be developed in the basic model to
describe the reduction of the reproductive capacity
or the unnatural increase in mortality, functions with
different arguments (concentrations of treatment
substances, temperature, etc.).
Finally, a general observation refers to the ethical
aspect of such research. The human species has
acquired, over time, an increasingly aggressive
character. Man tends to exterminate any species that
endangers his existence or affects his comfort. The
man decided to fight and destroy his opponent,
instead of learning to live with him. To the same
extent, other species have developed responses
corresponding to human actions. In this context, the
moral aspect of such research, in which living
beings are killed, is debatable. Creation has its laws,
and predator-prey behaviour seems to be one that
cannot be changed by man. Even though man tries
to tame this law, our species only manages to
simulate this action in reality, even in interpersonal
relationships, the prey-predator behaviour is the real
one.
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